Colorimetric detection of human papilloma virus by double isothermal amplification.
نویسندگان
چکیده
We developed a polymerase reaction free, low-cost and sensitive assay for the colorimetric detection of Human Papilloma Virus (HPV), based on the use of a smart design exploiting magnetic microbeads, chimeric RNA/DNAzyme oligonucleotides, and double signal amplification. This method allows obtaining a fast response with a detection limit of 10 pM, avoiding the amplification of the target via traditional PCR.
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ورودعنوان ژورنال:
- Chemical communications
دوره 49 90 شماره
صفحات -
تاریخ انتشار 2013